Fascination About attune flow cytometer
Fascination About attune flow cytometer
Blog Article
At that time, the early symptoms ended up dismissed as particles, even so the retrospective analysis shown shared characteristics Using the problematic cells in lifestyle. Tracing the an infection aided the lab build more laboratory processes for screening and defense of assay-significant mobile traces.
As samples are obtained over the Attune CytPix Flow Cytometer, the significant-pace brightfield camera captures and retailers pictures of detected occasions in a amount nearly six,000 pictures per next, according to the flow rate and graphic dimension. For increased versatility, Attune Cytometric Application helps you to change the graphic capture frequency as needed and choose a particular gate for graphic seize, if needed.
(B) Uncooked 264.7 macrophages are frequently annotated inaccurately with regard to particle rely due to their complexity and noncircular borders. The volume of singlets available for analysis boosts as soon as the user trains and employs a more accurate design.
Discover new insights into your experimental knowledge by visualizing populations utilizing put together fluorescence and morphology information. Our software program interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment which can be developed into your software program for an intuitive, streamlined facts Evaluation experience.
*Pricing is indicative only which is matter to alter. Be sure to Speak to your Agilent agent with any questions. The knowledge set forth herein is just not an offer or perhaps a commitment, both equally of which can be subject matter to credit score acceptance, stipulations, and availability with Agilent. Agilent Money Methods is accessible in chosen nations.
Here, a highly skilled person has gated singlets confidently. Immediately after assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate has in excess of four% aggregates.
Pair your Attune flow cytometer with the autosampler for increased workflow effectiveness, and include robotic integration for max throughput.
Our researchers will layout panels to suit your needs as repeatedly you would like—gratis, no merchandise obtain desired.
The Attune NxT cytometer could be the trusted workhorse instrument your lab can depend upon for regular and reliable effectiveness. The Attune CytPix model contains a substantial-speed brightfield digicam and AI-driven image Examination computer software, with current updates such as machine Studying instruments and an intuitive user interface, enabling enhanced information precision, accuracy, and speedier insights into sample biology.
Users can check out celebration photographs in authentic-time to confirm sample excellent, optimize protocols, and history the morphology of each and every mobile populace identified for Assessment. Imaging seize also benefits from acoustic focusing, which helps to situation the cells in order that a sharp, centered graphic is attained.
Audio waves permit precise mobile alignment, causing Extraordinary data quality and ~10X speedier sample flow rates.
We Beforehand demonstrated the power of imaging Car or truck-T/Ramos cell interactions. Enable’s evaluate just the populace of biggest fascination, the double beneficial gatherings, To find out more. We can now use extended morphological parameters (below, circularity and skewness of depth) to even more study the capabilities of these populations and refine gating on these activities, escalating info website robustness.
Leverage the strength of advanced technology and innovation to accelerate your discoveries. Belief inside the Attune Flow Cytometer family to deliver the trustworthiness, speed, and accuracy that established your operate aside.
No clean/no lyse leukocyte Investigation with imaging. Cells were acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Skilled UX/UI permits users to coach AI versions for expanded purposes. (A) The “Practice Design” characteristic presents annotation equipment and authentic-time design training accuracy.
At the guts of every Attune Flow Cytometer lies our groundbreaking acoustic focusing engineering, which utilizes audio waves to meticulously align cells throughout the flow mobile. This innovative method surpasses conventional hydrodynamic approaches, leading to a lot quicker processing speeds, Fantastic data high-quality, and enhanced sample throughput.
Because the mobile line had previously been analyzed within the Attune CytPix Flow Cytometer, the scientists went again to the pictures and were being in a position to doc the microbial infection at least 5 days before.
The Attune CytPix Flow Cytometer will allow end users to visualise samples all through acquisition, confirm the accuracy of gating methods, and guidance outcomes with reliable documentation for publication uses.
What appeared to be a homogeneous populace is in fact additional various—an insight that needs to be considered when interpreting effects.